Difference between revisions of "Part:BBa K3174002"

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This part has the blue fluorescent protein (mTagBFP) gene inserted downstream of a strong promoter and strong ribosome binding site.
 
This part has the blue fluorescent protein (mTagBFP) gene inserted downstream of a strong promoter and strong ribosome binding site.
 +
<partinfo>BBa_J23104</partinfo> is part of the Anderson Series of constitutive promoters designed by iGEM2006_Berkeley. It was used by Manchester in 2017 to create <partinfo>BBa_K2213008</partinfo>.
  
  

Revision as of 04:46, 19 August 2019


Blue Fluorescent Protein with Strong Promoter and Strong RBS

This part has the blue fluorescent protein (mTagBFP) gene inserted downstream of a strong promoter and strong ribosome binding site. BBa_J23104 is part of the Anderson Series of constitutive promoters designed by iGEM2006_Berkeley. It was used by Manchester in 2017 to create BBa_K2213008.


Usage and Biology

UT Austin iGEM 2019 Team transformed this part into the E.coli DH10B burden monitor (https://2019.igem.org/Team:Austin_UTexas
) and used it as a standard with which cellular burden could be quantified (by means of a GFP expression vs growth rate model) for other BioBricks that had been similarly transformed into the burden strain.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]