Difference between revisions of "Part:BBa K3174007"
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This part has the blue fluorescent protein (mTagBFP) gene inserted behind a medium promoter and weak ribosome binding site. | This part has the blue fluorescent protein (mTagBFP) gene inserted behind a medium promoter and weak ribosome binding site. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | UT Austin iGEM 2019 Team transformed this part into the E.coli DH10B burden monitor and used it as a standard with which cellular burden could be quantified (by means of a GFP expression vs growth rate model) for other biobricks that had been similarly transformed into the burden strain. | ||
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Revision as of 20:09, 8 August 2019
Blue Fluorescent Protein with Medium Promoter and Weak RBS
This part has the blue fluorescent protein (mTagBFP) gene inserted behind a medium promoter and weak ribosome binding site.
Usage and Biology
UT Austin iGEM 2019 Team transformed this part into the E.coli DH10B burden monitor and used it as a standard with which cellular burden could be quantified (by means of a GFP expression vs growth rate model) for other biobricks that had been similarly transformed into the burden strain.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]