Difference between revisions of "Part:BBa K2982000:Design"
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===Source=== | ===Source=== | ||
| − | Sequence of wild type PETase optimized for Escherichia coli from ' | + | Sequence of wild type PETase optimized for Escherichia coli from 'Characterization and engineering of a plastic-degrading aromatic polyesterase', PNAs, 2018. |
===References=== | ===References=== | ||
Joo, S., Cho, I. J., Seo, H., Son, H. F., Sagong, H., Shin, T. J., . . . Kim, K. (2018). Structural insight into molecular mechanism of poly(ethylene terephthalate) degradation. Nature Communications, 9(1). doi:10.1038/s41467-018-02881-1 | Joo, S., Cho, I. J., Seo, H., Son, H. F., Sagong, H., Shin, T. J., . . . Kim, K. (2018). Structural insight into molecular mechanism of poly(ethylene terephthalate) degradation. Nature Communications, 9(1). doi:10.1038/s41467-018-02881-1 | ||
Revision as of 04:35, 7 August 2019
Coding sequence for wild type IsPETase
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 348
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 304
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 348
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 348
Illegal AgeI site found at 627 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Extra amino acids are added to the N-terminus and C-terminus of the codon optimized DNA sequence used for expression of wild type Ideonella sakainesis PETase.
Source
Sequence of wild type PETase optimized for Escherichia coli from 'Characterization and engineering of a plastic-degrading aromatic polyesterase', PNAs, 2018.
References
Joo, S., Cho, I. J., Seo, H., Son, H. F., Sagong, H., Shin, T. J., . . . Kim, K. (2018). Structural insight into molecular mechanism of poly(ethylene terephthalate) degradation. Nature Communications, 9(1). doi:10.1038/s41467-018-02881-1