Difference between revisions of "Part:BBa K2982000"
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<partinfo>BBa_K2982000 short</partinfo> | <partinfo>BBa_K2982000 short</partinfo> | ||
| − | A coding sequence for wild type PETase from Ideonella sakainesis which is in BioBrick RFC[10] assembly standard and also contains two site for restriction enzymes, XhoI and BamHI to act on. It is codon optimized for Escherichia coli. Can serve as a standard to | + | A coding sequence for wild type PETase from Ideonella sakainesis which is in BioBrick RFC[10] assembly standard and also contains two site for restriction enzymes, XhoI and BamHI to act on. It is codon optimized for Escherichia coli. Can serve as a standard for other experiment related to PETase. |
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Revision as of 12:30, 3 August 2019
Coding sequence for wild type IsPETase
A coding sequence for wild type PETase from Ideonella sakainesis which is in BioBrick RFC[10] assembly standard and also contains two site for restriction enzymes, XhoI and BamHI to act on. It is codon optimized for Escherichia coli. Can serve as a standard for other experiment related to PETase.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 348
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 304
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 348
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 348
Illegal AgeI site found at 627 - 1000COMPATIBLE WITH RFC[1000]