Difference between revisions of "Part:BBa K2916013:Design"
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
  
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The sequence was extracted from the pET21a-AsnRS-His plasmid and expressed in the BL21 (DE3) E.coli strain.
+
The sequence was extracted from the pET21a-ACysRS-His plasmid and expressed in the BL21 (DE3) E.coli strain.
  
 
===Source===
 
===Source===

Revision as of 11:06, 24 July 2019


GlyRS protein equipped with a 6x HIS affinity tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2989
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1737
    Illegal AgeI site found at 471
    Illegal AgeI site found at 2859
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 958


Design Notes

The sequence was extracted from the pET21a-ACysRS-His plasmid and expressed in the BL21 (DE3) E.coli strain.

Source

http://www.addgene.org/124106/

References

Cell-free translation reconstituted with purified components. Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, Ueda T. Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802. 10.1038/90802 PubMed 11479568