Difference between revisions of "Part:BBa J119440"

Revision as of 21:23, 18 July 2019

c-Myc Promoter G-quadruplex and i-Motif

The part forms an i-motif and/or G quadruplex, and has sticky ends on both 5' ends. The i-motif is located on the template strand in this sequence. If the part was to be ligated into a plasmid that had been digested with Eco RI, it could be ligated in either oreintation (i-motif on the coding strand or G-quadruplex on the coding strand). The i-motif/G-quadruplex-forming sequence comes from the human c-Myc promoter's Nuclease Hypersenstive Element III. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_J119440 short</partinfo>
-The part forms an i-motif and/or G quadruplex, and has sticky ends on both 5' ends. These ends allow the part to be inserted into an Eco RI cut site with either strand on the template or coding strand. The G-quadruplex is shown on the coding strand in this sequence. The i-motif/G-quadruplex-forming sequence comes from the human c-Myc promoter's Nuclease Hypersenstive Element III.  If the part was to be ligated into a plasmid that had been digested with Eco RI, it could be ligated in either oreintation (i-motif on the coding strand or G-quadruplex on the coding strand).
+The part forms an i-motif and/or G quadruplex, and has sticky ends on both 5' ends. The i-motif is located on the template strand in this sequence.  If the part was to be ligated into a plasmid that had been digested with Eco RI, it could be ligated in either oreintation (i-motif on the coding strand or G-quadruplex on the coding strand).  The i-motif/G-quadruplex-forming sequence comes from the human c-Myc promoter's Nuclease Hypersenstive Element III.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===