Difference between revisions of "Part:BBa K3182000"

Revision as of 17:09, 11 July 2019

pT7-CBDcipA-AsPink

A cellulose binding domain (CBD) which can be used to purify or attach proteins to cellulose. The part also has a flexible GS-linker with a thrombin site added at the end, clevage with thrombin will add one glycine and one serine to the start of the C-terminal of the fusion tail. The part has a AsPink fused to the CBD to ensure easy trackable expression and characterization of the CBD. The part has a very strong expression with a T7 promotor as well as a 5'UTR region which has been shown to further increase expression in E. coli.

Figure 1. E. coli BL21 cells expressing this biobrick, incubated for 16 hours at 16 degrees Celsius.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 580
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3182000 short</partinfo>
-A cellulose binding domain which can be used to purify or attach proteins to cellulose. The part also has a flexible GS-linker with a thrombin site added. The part has a AsPink fused to the cellulose binding domain to ensure easy trackable expression and characterization. The part has the strongest available expression system with a T7 promotor and 5'-UTR region as well as other translational enhancers.
+A cellulose binding domain (CBD) which can be used to purify or attach proteins to cellulose. The part also has a flexible GS-linker with a thrombin site added at the end, clevage with thrombin will add one glycine and one serine to the start of the C-terminal of the fusion tail. The part has a AsPink fused to the CBD to ensure easy trackable expression and characterization of the CBD. The part has a very strong expression with a T7 promotor as well as a 5'UTR region which has been shown to further increase expression in E. coli.
 [[File:T--Linkoping_Sweden--CBD-AsPink.jpeg|430px|thumb|center|<b>Figure 1.</b> E. coli BL21 cells expressing this biobrick, incubated for 16 hours at 16 degrees Celsius.]]