Difference between revisions of "Part:BBa K2905013"

 
 
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<partinfo>BBa_K2905013 short</partinfo>
 
<partinfo>BBa_K2905013 short</partinfo>
  
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Pyrrolysine--tRNA ligase PylS, under control of J23101 and B0034. Contains 6 His tag and TEV protease site.
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Pyrrolysine--tRNA ligase (PylS) under control of constitutive promoter (J23101) and ribosome binding site (B0034). Contains 6 His tag and TEV protease site.
  
 
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Latest revision as of 19:29, 24 June 2019


Constitutively expressed 6-His-TEV-PylS

Pyrrolysine--tRNA ligase (PylS) under control of constitutive promoter (J23101) and ribosome binding site (B0034). Contains 6 His tag and TEV protease site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 104
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]