Difference between revisions of "Part:BBa K2905011:Design"

 
(Design Notes)
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===Design Notes===
 
===Design Notes===
Codon optimized. 3A assembly scar sites included between J23101, RBS, and Coding region.
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Codon optimized; 3A assembly scar sites included between promoter, RBS, and coding region.
 
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===Source===
 
===Source===

Revision as of 19:26, 24 June 2019


Constitutively expressed 6-His-TEV-PylD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 104
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 139
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized; 3A assembly scar sites included between promoter, RBS, and coding region.

Source

IDT synthesized DNA, codon optimized.

References