Difference between revisions of "Part:BBa K2905009:Design"
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Latest revision as of 19:21, 24 June 2019
Constitutively expressed 6-His-TEV-PylC
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 104 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 214
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimized; 3A assembly scar sites included between promoter, RBS, and coding region.
Source
IDT synthesized DNA.