Difference between revisions of "Part:BBa K2448001"
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<partinfo>BBa_K2448001 short</partinfo> | <partinfo>BBa_K2448001 short</partinfo> | ||
| − | mEmerald is a derivative of the Green Fluorescent Protein from ''Aequorea victoria'' with improved features in terms of intrinsic brightness, photostability, resistance to bleaching, maturation and folding at 37°C [1]. The protein contains | + | mEmerald is a derivative of the Green Fluorescent Protein from ''Aequorea victoria'' with improved features in terms of intrinsic brightness, photostability, resistance to bleaching, maturation and folding at 37°C [1]. The protein contains several mutations compared to wild-type (Uniprot P42212): +2V, F64L, S65T, S72A, N149K, M153T, I167T, A206K and H232L. |
Figure 1 illustrates the intense colour of mEmerald expressing ''E. coli'' cells. | Figure 1 illustrates the intense colour of mEmerald expressing ''E. coli'' cells. | ||
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Figure 1. mEmerald expression in ''E. coli'' cells harbouring [[Part:BBa_K2448023|BBa_K2448023]]. Transformed celle were plated on a LB plate containing 35 µg/mL chloramphenicol (no IPTG, no X-gal). | Figure 1. mEmerald expression in ''E. coli'' cells harbouring [[Part:BBa_K2448023|BBa_K2448023]]. Transformed celle were plated on a LB plate containing 35 µg/mL chloramphenicol (no IPTG, no X-gal). | ||
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| + | ===References=== | ||
| + | |||
| + | [1] Cubitt AB, Woollenweber LA, Heim R. Understanding structure-function relationships in the ''Aequorea victoria'' green fluorescent protein. Methods Cell Biol. (1999) 58, 19–30. | ||
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Latest revision as of 19:06, 31 December 2018
mEmerald
mEmerald is a derivative of the Green Fluorescent Protein from Aequorea victoria with improved features in terms of intrinsic brightness, photostability, resistance to bleaching, maturation and folding at 37°C [1]. The protein contains several mutations compared to wild-type (Uniprot P42212): +2V, F64L, S65T, S72A, N149K, M153T, I167T, A206K and H232L.
Figure 1 illustrates the intense colour of mEmerald expressing E. coli cells.
Usage and Biology
Applications are various: Fusions, promoter & secretion studies, FRET and other quantitative imaging techniques.
Our team used this codon optimized mEmerald to build the Universal Biosensing Chassis (BBa_K2448023).
Figure 1. mEmerald expression in E. coli cells harbouring BBa_K2448023. Transformed celle were plated on a LB plate containing 35 µg/mL chloramphenicol (no IPTG, no X-gal).
References
[1] Cubitt AB, Woollenweber LA, Heim R. Understanding structure-function relationships in the Aequorea victoria green fluorescent protein. Methods Cell Biol. (1999) 58, 19–30.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 28
Illegal AgeI site found at 151 - 1000COMPATIBLE WITH RFC[1000]