Difference between revisions of "Part:BBa K2448054"
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<partinfo>BBa_K2448054 short</partinfo> | <partinfo>BBa_K2448054 short</partinfo> | ||
− | This part is an E. coli codon optimized version of the D-Psicose 3-epimerase (Dpe) from Clostridium cellulolyticum str. ATCC 35319 (Uniprot B8I944) with a C-terminal HisTag | + | This part is an ''E. coli'' codon optimized version of the D-Psicose 3-epimerase (Dpe) from ''Clostridium cellulolyticum'' str. ATCC 35319 |
+ | (Uniprot B8I944) with a C-terminal HisTag [[Part:BBa_K2448053|BBa_K2448053]]) equipped with a strong RBS ([[Part:BBa_K2448019|BBa_K2448019]]) and placed under the control of the pTacI promoter ([[Part:BBa_K864400|BBa_K864400]]). | ||
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Revision as of 13:30, 27 December 2018
D-Psicose 3-epimerase (Dpe) from Clostridium cellulolyticum with a C-terminal HisTag under the contr
This part is an E. coli codon optimized version of the D-Psicose 3-epimerase (Dpe) from Clostridium cellulolyticum str. ATCC 35319 (Uniprot B8I944) with a C-terminal HisTag BBa_K2448053) equipped with a strong RBS (BBa_K2448019) and placed under the control of the pTacI promoter (BBa_K864400).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 966
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 714
- 1000COMPATIBLE WITH RFC[1000]