Difference between revisions of "Part:BBa M50470:Design"
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
We added an aeBlue chromoprotein sequence with an RBS taken from Bba_K328000 downstream of our cspA promoter (Bba_K1895002) such that the cspA promoter controls transcription of the aeBlue chromoprotein. Behind the chromoprotein sequence is a 3X-FLAG epitope tag (Bba_K823034) that we can use for Western blotting and other protein separation experiments.
+
We added an aeBlue chromoprotein sequence with an RBS taken from BBa_K1033929 downstream of our cspA promoter (Bba_K328000) such that the cspA promoter controls transcription of the aeBlue chromoprotein. Behind the chromoprotein sequence is a 3X-FLAG epitope tag (Bba_K823034) that we can use for Western blotting and other protein separation experiments.
  
 
===Source===
 
===Source===
  
−
Bba_K328000
+
aeBlue chromoprotein: BBa_K1033929
−
Bba_K1895002
+
cspA promoter:  Bba_K328000
−
Bba_K823034
+
3X-FLAG tag: Bba_K823034
  
  
 
===References===
 
===References===
 +
https://parts.igem.org/Part:BBa_K328000
 +
https://parts.igem.org/Part:BBa_K1033929
 +
https://parts.igem.org/Part:BBa_K823034

Revision as of 13:42, 12 December 2018


Cold Shock Sensor w/ aeBlue+3X-FLAG tag Pigment Reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We added an aeBlue chromoprotein sequence with an RBS taken from BBa_K1033929 downstream of our cspA promoter (Bba_K328000) such that the cspA promoter controls transcription of the aeBlue chromoprotein. Behind the chromoprotein sequence is a 3X-FLAG epitope tag (Bba_K823034) that we can use for Western blotting and other protein separation experiments.

Source

aeBlue chromoprotein: BBa_K1033929 cspA promoter: Bba_K328000 3X-FLAG tag: Bba_K823034


References

https://parts.igem.org/Part:BBa_K328000 https://parts.igem.org/Part:BBa_K1033929 https://parts.igem.org/Part:BBa_K823034