Difference between revisions of "Part:BBa M50477:Design"

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===References===
 
===References===
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https://parts.igem.org/Part:BBa_K1895002
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https://parts.igem.org/Part:BBa_K1033922
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https://parts.igem.org/Part:BBa_BBa_K112703
  
 
Agashe, V. R., & Hartl, F. (2000). Roles of molecular chaperones in cytoplasmic protein folding. Seminars in Cell & Developmental Biology, 11(1), 15-25.  
 
Agashe, V. R., & Hartl, F. (2000). Roles of molecular chaperones in cytoplasmic protein folding. Seminars in Cell & Developmental Biology, 11(1), 15-25.  
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Rodriguez JL, et al. 2014. Selection of Escherichia coli heat shock promoters toward their application as stress probes. J Biotechnol. 188:61-71.
 
Rodriguez JL, et al. 2014. Selection of Escherichia coli heat shock promoters toward their application as stress probes. J Biotechnol. 188:61-71.
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===Stanford Location===
 
===Stanford Location===

Latest revision as of 01:49, 12 December 2018

Heat-shock-indued chromoprotein expression plasmid (pHeat)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 547

Design Notes

Our pHeat construct begins with heat shock promoter, hsp70 (BBa_K1895002) that will induce the expression of red chromoprotein, meffRed (BBa_K1033922), at higher temperatures. We chose this specific heat-shock promoter because it has been the most studied and has been shown to be more effective at sensing hot temperatures. meffRed is a bright red chromoprotein that comes from the coral Montipora efflorescens; this part already contain an RBS. It was chosen because of its relatively short length and the fact that it already contains a ribosome binding site.To better quantify the amount of meffRed produced during experimentation, we included a 6x-His tag (BBa_K112703) on the meffRed chromoprotein, in order to allow for Western blot analyses, if necessary.

Basic Parts

Hsp70 promoter: BBa_K1895002

meffRed: BBa_K1033922

6x-His tag: BBa_K112703

References

https://parts.igem.org/Part:BBa_K1895002

https://parts.igem.org/Part:BBa_K1033922

https://parts.igem.org/Part:BBa_BBa_K112703

Agashe, V. R., & Hartl, F. (2000). Roles of molecular chaperones in cytoplasmic protein folding. Seminars in Cell & Developmental Biology, 11(1), 15-25.

Arsène F, Tomoyasu T, Bukau B. The heat shock response of Escherichia coli. Int J Food Microbiol. 2000 Apr 10;55(1-3):3-9.

Rodriguez JL, et al. 2014. Selection of Escherichia coli heat shock promoters toward their application as stress probes. J Biotechnol. 188:61-71.

Stanford Location

Plasmid name: pHeat

Organism: E. coli

Device type: Sensor

Glycerol stock barcode: 0133012020

Box label: BioE44 F18