Difference between revisions of "Part:BBa M50477:Design"
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===Basic Parts=== | ===Basic Parts=== | ||
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Hsp70 promoter: BBa_K1895002 | Hsp70 promoter: BBa_K1895002 | ||
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meffRed: BBa_K1033922 | meffRed: BBa_K1033922 | ||
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| + | 6x-His tag: BBa_K823035 | ||
===References=== | ===References=== | ||
Revision as of 01:16, 12 December 2018
Heat-shock-indued chromoprotein expression plasmid (pHeat)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 547
Design Notes
Our pHeat construct begins with heat shock promoter, hsp70 , that will induce the expression of red chromoprotein, meffRed, at higher temperatures. To better quantify the amount of meffRed produced during experimentation, we included a 6x-His tag on the meffRed chromoprotein, in order to allow for Western blot analyses, if necessary. hsp70 is a constitutive heat-shock promoter listed as part BBa_K1895002. We chose this specific heat-shock promoter because it has been the most studied and has been shown to be more effective at sensing hot temperatures. meffRed (part BBa_K1033922) is a bright red chromoprotein that comes from the coral Montipora efflorescens. It was chosen because of its relatively short length and the fact that it already contains a ribosome binding site.
Basic Parts
Hsp70 promoter: BBa_K1895002
meffRed: BBa_K1033922
6x-His tag: BBa_K823035