Difference between revisions of "Part:BBa M50477:Design"
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<partinfo>BBa_M50477 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_M50477 SequenceAndFeatures</partinfo>
  
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===Design Notes===
 
Our pHeat construct begins with heat shock promoter, hsp70 , that will induce the expression of red chromoprotein, meffRed, at higher temperatures. To better quantify the amount of meffRed produced during experimentation, we included a 6x-His tag on the meffRed chromoprotein, in order to allow for Western blot analyses, if necessary. hsp70 is a constitutive heat-shock promoter listed as part BBa_K1895002. We chose this specific heat-shock promoter because it has been the most studied and has been shown to be more effective at sensing hot temperatures. meffRed (part BBa_K1033922) is a bright red chromoprotein that comes from the coral Montipora efflorescens. It was chosen because of its relatively short length and the fact that it already contains a ribosome binding site.
 
Our pHeat construct begins with heat shock promoter, hsp70 , that will induce the expression of red chromoprotein, meffRed, at higher temperatures. To better quantify the amount of meffRed produced during experimentation, we included a 6x-His tag on the meffRed chromoprotein, in order to allow for Western blot analyses, if necessary. hsp70 is a constitutive heat-shock promoter listed as part BBa_K1895002. We chose this specific heat-shock promoter because it has been the most studied and has been shown to be more effective at sensing hot temperatures. meffRed (part BBa_K1033922) is a bright red chromoprotein that comes from the coral Montipora efflorescens. It was chosen because of its relatively short length and the fact that it already contains a ribosome binding site.
  
  
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===Basic Parts===
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Hsp70 promoter: BBa_K1895002
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meffRed: BBa_K1033922
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6x-His tag: BBa_K823035
  
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BBa_K1895002
 
  
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===References===
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References
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Revision as of 01:16, 12 December 2018

Heat-shock-indued chromoprotein expression plasmid (pHeat)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 547

Design Notes

Our pHeat construct begins with heat shock promoter, hsp70 , that will induce the expression of red chromoprotein, meffRed, at higher temperatures. To better quantify the amount of meffRed produced during experimentation, we included a 6x-His tag on the meffRed chromoprotein, in order to allow for Western blot analyses, if necessary. hsp70 is a constitutive heat-shock promoter listed as part BBa_K1895002. We chose this specific heat-shock promoter because it has been the most studied and has been shown to be more effective at sensing hot temperatures. meffRed (part BBa_K1033922) is a bright red chromoprotein that comes from the coral Montipora efflorescens. It was chosen because of its relatively short length and the fact that it already contains a ribosome binding site.


Basic Parts

Hsp70 promoter: BBa_K1895002 meffRed: BBa_K1033922 6x-His tag: BBa_K823035


References