Difference between revisions of "Part:BBa M50477"

 
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<partinfo>BBa_M50477 short</partinfo>
 
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This plasmid sense warm temperatures and produces red pigment (meffRed) when activated. The warmer the temperature, the more red pigment that is produced.
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This plasmid senses warm temperatures using the heat-shock protein 70 promoter (hsp70) and produces red pigment (meffRed) when activated. The warmer the temperature, the more red pigment that is produced. meffRed is tagged with a 6X-His tag, which facilitates Western blot analysis of meffRed production.
  
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Dynamic range experimentation concluded that no meffRed is visibly produced after heat-shock at a variety of warm temperatures after 60 minutes; Western blot analysis also confirmed these results. Future experimentation could look at reverse transcription analysis to see if mRNA is being produced after heat-shock. If not, utilizing a composite part with a different heat-shock promoter is recommended.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_M50477 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_M50477 parameters</partinfo>
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Revision as of 01:10, 12 December 2018


Heat-shock-indued chromoprotein expression plasmid (pHeat)

This plasmid senses warm temperatures using the heat-shock protein 70 promoter (hsp70) and produces red pigment (meffRed) when activated. The warmer the temperature, the more red pigment that is produced. meffRed is tagged with a 6X-His tag, which facilitates Western blot analysis of meffRed production.

Dynamic range experimentation concluded that no meffRed is visibly produced after heat-shock at a variety of warm temperatures after 60 minutes; Western blot analysis also confirmed these results. Future experimentation could look at reverse transcription analysis to see if mRNA is being produced after heat-shock. If not, utilizing a composite part with a different heat-shock promoter is recommended.