Difference between revisions of "Part:BBa M50499:Design"

 
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===Design Notes===
 
===Design Notes===
 +
 +
 
We were unsure whether to put the CRP binding site immediately downstream or with a buffer sequence. We ended up putting it right after the promoter.
 
We were unsure whether to put the CRP binding site immediately downstream or with a buffer sequence. We ended up putting it right after the promoter.
  

Revision as of 23:48, 11 December 2018


Catabolite Activator Protein (CAP) Repressed Promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1059
    Illegal PstI site found at 688
    Illegal PstI site found at 849
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 144
    Illegal NheI site found at 167
    Illegal PstI site found at 688
    Illegal PstI site found at 849
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1059
    Illegal PstI site found at 688
    Illegal PstI site found at 849
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1059
    Illegal PstI site found at 688
    Illegal PstI site found at 849
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We were unsure whether to put the CRP binding site immediately downstream or with a buffer sequence. We ended up putting it right after the promoter.


Source

IGEM part: BBa_S05450 = constitutive promoter IGEM part: BBa_M36547 = CRP binding site

Paper: www.ncbi.nlm.nih.gov/pmc/articles/PMC338411/

References