Difference between revisions of "Part:BBa M50499"

 
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<partinfo>BBa_M50499 short</partinfo>
 
<partinfo>BBa_M50499 short</partinfo>
  
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This part is a constitutive promoter followed by the cAMP receptor protein (CRP) downstream from this promoter. The idea is that the CRP-bound operator would block RNA polymerase from transcribing.
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We placed the constitutive promoter (BBa_S05450, iGEM) immediately upstream from a CAP binding site (BBa_M36547, iGEM) in Plasmid E.  
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For our experiments, we placed this part upstream from GFP in order to assay expression levels through fluorescent intensity.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 23:39, 11 December 2018


Catabolite Activator Protein (CAP) Repressed Promoter

We placed the constitutive promoter (BBa_S05450, iGEM) immediately upstream from a CAP binding site (BBa_M36547, iGEM) in Plasmid E.

For our experiments, we placed this part upstream from GFP in order to assay expression levels through fluorescent intensity.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1059
    Illegal PstI site found at 688
    Illegal PstI site found at 849
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 144
    Illegal NheI site found at 167
    Illegal PstI site found at 688
    Illegal PstI site found at 849
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1059
    Illegal PstI site found at 688
    Illegal PstI site found at 849
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1059
    Illegal PstI site found at 688
    Illegal PstI site found at 849
  • 1000
    COMPATIBLE WITH RFC[1000]