Difference between revisions of "Part:BBa K2624005:Experience"
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===Applications of BBa_K2624005=== | ===Applications of BBa_K2624005=== | ||
| − | We used this part to construct the "p-H" Plasmid and "Plasmid2", in order to verify role of RNA interference caused by pri-miRNA. According to the results of qPCR, the decreased MAP4K4 mRNA expression was observed. What's more, the results of MTT shows that the regulatory system we | + | We used this part to construct the "p-H" Plasmid and "Plasmid2", in order to verify the role of RNA interference caused by pri-miRNA. According to the results of qPCR, the decreased MAP4K4 mRNA expression was observed. What's more, the results of MTT shows that the regulatory system we built can work. |
https://static.igem.org/mediawiki/2018/archive/5/56/20181017211354%21T--CPU_CHINA--hp-qidongzi.png | https://static.igem.org/mediawiki/2018/archive/5/56/20181017211354%21T--CPU_CHINA--hp-qidongzi.png | ||
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Figure 2. The MTT Assay | Figure 2. The MTT Assay | ||
| − | "Plasmid1" | + | "Plasmid1" contains "tTA-NS5B-NLS" parts; "Plasmid2" contains "HULC--primiRNA(MAP4K4)-U6-pSil-TRE-sponge" parts; TC is the abbreviations of Tetracycline. |
===User Reviews=== | ===User Reviews=== | ||
Revision as of 03:46, 8 December 2018
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K2624005
We used this part to construct the "p-H" Plasmid and "Plasmid2", in order to verify the role of RNA interference caused by pri-miRNA. According to the results of qPCR, the decreased MAP4K4 mRNA expression was observed. What's more, the results of MTT shows that the regulatory system we built can work.
Figure 1. The expression of MAP4K4 mRNA
"p-H" is "pcDNA-HULC--primiRNA(MAP4K4)" plasmid;"Plasmid1" is"pIRES-tTA-NS5B-NLS";"Plasmid2" is"pcDNA-HULC--primiRNA(MAP4K4)-U6-pSil-TRE-sponge".
Figure 2. The MTT Assay
"Plasmid1" contains "tTA-NS5B-NLS" parts; "Plasmid2" contains "HULC--primiRNA(MAP4K4)-U6-pSil-TRE-sponge" parts; TC is the abbreviations of Tetracycline.
User Reviews
UNIQ054bb92a22f33efe-partinfo-00000000-QINU UNIQ054bb92a22f33efe-partinfo-00000001-QINU