Difference between revisions of "Part:BBa K2624000:Experience"

Latest revision as of 03:33, 8 December 2018

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2624000

To improve the selectivity of our system, we utilized hTERT, the cancer-specific promoter, along with HULC, a HCC(Hepatocellular carcinoma)-specific promoter, to open an "AND" gate system to target HCC. We used pGL3-Basic vector, a promoter-less vector for the luciferase assay to determine the transcriptional activity of these promoters. We added the promoters on pGL3-Basic vector and measure the OD value of the luciferase activity. We chose SV40, a highly activated promoter in both cancer cells and normal cells as positive control (PGL3-CON). Results show that hTERT promoter has a good efficiency in cancer cells.

20181017205901%21T--CPU_CHINA--hp-qidongzi.png

Figure. The activity of the promoters

User Reviews

UNIQ61528bd0b382173e-partinfo-00000000-QINU UNIQ61528bd0b382173e-partinfo-00000001-QINU

@@ Line 5: / Line 5: @@
 ===Applications of BBa_K2624000===
-To improve the selectivity of our system, we utilize hTERT, the cancer-specific promoter, along with HULC, a HCC(Hepatocellular carcinoma)-specific promoter, to open an "AND" gate system to target HCC. We use pGL3-Basic vector, a promoter-less vector for the luciferase assay to determine the transcriptional activity of these promoters. We add the promoters on pGL3-Basic vector and measure the OD value of the luciferase activity. We choose SV40, a highly activated promoter in both cancer cells and normal cells as positive control (PGL3-CON). Results show that hTERT promoter has a good efficiency in cancer cells.
+To improve the selectivity of our system, we utilized hTERT, the cancer-specific promoter, along with HULC, a HCC(Hepatocellular carcinoma)-specific promoter, to open an "AND" gate system to target HCC. We used pGL3-Basic vector, a promoter-less vector for the luciferase assay to determine the transcriptional activity of these promoters. We added the promoters on pGL3-Basic vector and measure the OD value of the luciferase activity. We chose SV40, a highly activated promoter in both cancer cells and normal cells as positive control (PGL3-CON). Results show that hTERT promoter has a good efficiency in cancer cells.
 https://static.igem.org/mediawiki/2018/archive/5/56/20181017205901%21T--CPU_CHINA--hp-qidongzi.png