Difference between revisions of "Part:BBa K2832004"

Revision as of 04:17, 7 December 2018

PzntA - Inducible Promoter

The zntAp promoter upregulates the zntA gene for the ATPase zinc exporter, ZntA. To decrease high intracellular concentrations of zinc, the zntA gene expresses ZntA upon activation by the cytosolic zinc-bound transcription factor, ZntR. Zinc ions can enter the cell through mechanosensitive channels (Mscs) that are opened by a stress on the membrane, and can then bind to ZntR. The zntAp promoter is therefore indirectly activated by stress, in which four zinc ions must enter the cell through the Msc to bind and activate ZntR. Our system incorporates a region between -35 and -10 b.p. upstream of the zntAp promoter for the binding of zinc-bound ZntR for mRFP upregulation. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 13
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2832004 short</partinfo>
-The zntAp promoter upregulates the zntA gene for the ATPase zinc exporter, ZntA. To decrease high intracellular concentrations of zinc, the zntA gene expresses ZntA upon activation by the cytosolic zinc-bound transcription factor, ZntR. Zinc ions can enter the cell through mechanosensitive channels (mscs) that are opened by a stress on the membrane, and can then bind to ZntR. The zntAp promoter is therefore indirectly activated by stress, in which four zinc ions must enter the cell through the msc to bind and activate ZntR. Our system incorporates a region between -35 and -10 b.p. upstream of the zntAp promoter for the binding of zinc-bound ZntR for mRFP upregulation.
+The zntAp promoter upregulates the zntA gene for the ATPase zinc exporter, ZntA. To decrease high intracellular concentrations of zinc, the zntA gene expresses ZntA upon activation by the cytosolic zinc-bound transcription factor, ZntR. Zinc ions can enter the cell through mechanosensitive channels (Mscs) that are opened by a stress on the membrane, and can then bind to ZntR. The zntAp promoter is therefore indirectly activated by stress, in which four zinc ions must enter the cell through the Msc to bind and activate ZntR. Our system incorporates a region between -35 and -10 b.p. upstream of the zntAp promoter for the binding of zinc-bound ZntR for mRFP upregulation.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===