Difference between revisions of "Part:BBa K2832001"
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<partinfo>BBa_K2832001 short</partinfo> | <partinfo>BBa_K2832001 short</partinfo> | ||
| − | The OsmCp1 promoter upregulates the osmC gene for the cytosolic peroxiredoxin, OsmC. The function of this peroxiredoxin is to protect the cell from oxidative stress by organic hydroperoxides, and it is activated by stress from osmotic pressure. The OsmCp1 promoter is therefore stress-activated by transcription factors RcsB and NhaR. RcsB is a cytosolic regulator in the Rcs pathway that responds to stress on the plasma membrane by regulating capsular polysaccharide synthesis | + | The OsmCp1 promoter upregulates the osmC gene for the cytosolic peroxiredoxin, OsmC. The function of this peroxiredoxin is to protect the cell from oxidative stress by organic hydroperoxides, and it is activated by stress from osmotic pressure. The OsmCp1 promoter is therefore stress-activated by transcription factors RcsB and NhaR. RcsB is a cytosolic regulator in the Rcs pathway that responds to stress on the plasma membrane by regulating capsular polysaccharide synthesis as well as regulating vital cellular functions. NhaR responds to osmotic shock and high ionic osmolyte concentration. A region within the OsmCp1 promoter of -191 b.p. before the transcription start site was selected for the binding of RcsB and NhaR to activate mRFP. |
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Revision as of 05:29, 3 December 2018
PosmC - Inducible Promoter
The OsmCp1 promoter upregulates the osmC gene for the cytosolic peroxiredoxin, OsmC. The function of this peroxiredoxin is to protect the cell from oxidative stress by organic hydroperoxides, and it is activated by stress from osmotic pressure. The OsmCp1 promoter is therefore stress-activated by transcription factors RcsB and NhaR. RcsB is a cytosolic regulator in the Rcs pathway that responds to stress on the plasma membrane by regulating capsular polysaccharide synthesis as well as regulating vital cellular functions. NhaR responds to osmotic shock and high ionic osmolyte concentration. A region within the OsmCp1 promoter of -191 b.p. before the transcription start site was selected for the binding of RcsB and NhaR to activate mRFP.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 13
- 1000COMPATIBLE WITH RFC[1000]