Difference between revisions of "Part:BBa K2832000"
 
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<partinfo>BBa_K2832000 short</partinfo>
 
<partinfo>BBa_K2832000 short</partinfo>
  
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The bamEp promoter upregulates the bamE gene which is responsible for the aggregation of outer membrane proteins and membrane permeability by production of protein BamE. This promoter is activated by RpoE, the sigma 24 factor of RNA polymerase. Since RpoE is activated by external stress on the plasma membrane, it activates regulatory genes such as bamE to respond to extracellular stress. Stresses include misfolding, heat shock, and osmotic shock of outer membrane proteins, and structural changes to the membranous lipopolysaccharides. RpoE binds to bamEp within -41 b.p. before the transcription start site for our system.  
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The bamEp promoter upregulates the bamE gene which is responsible for the aggregation of outer membrane proteins and membrane permeability by production of protein BamE. This promoter is activated by RpoE, the sigma 24 factor of RNA polymerase. Since RpoE is activated by external stress on the plasma membrane, it activates regulatory genes such as bamE to respond to extracellular stress. Known stresses include misfolding, heat shock, and osmotic shock of outer membrane proteins, and structural changes to the membranous lipopolysaccharides. RpoE binds to bamEp within -41 b.p. before the transcription start site for upregulation of mRFP in our system.  
  
 
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Latest revision as of 05:26, 3 December 2018


PbamE - Inducible Promoter

The bamEp promoter upregulates the bamE gene which is responsible for the aggregation of outer membrane proteins and membrane permeability by production of protein BamE. This promoter is activated by RpoE, the sigma 24 factor of RNA polymerase. Since RpoE is activated by external stress on the plasma membrane, it activates regulatory genes such as bamE to respond to extracellular stress. Known stresses include misfolding, heat shock, and osmotic shock of outer membrane proteins, and structural changes to the membranous lipopolysaccharides. RpoE binds to bamEp within -41 b.p. before the transcription start site for upregulation of mRFP in our system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 13
  • 1000
    COMPATIBLE WITH RFC[1000]