Difference between revisions of "Part:BBa K2832015:Design"
 
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===Design Notes===
 
===Design Notes===
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https://static.igem.org/mediawiki/parts/4/44/T--PSB1C3-_apFAB82-mRFP1_map.png
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https://static.igem.org/mediawiki/parts/8/80/ApFAB82_Design_Diagram.png
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apFAB82 Test Device:  The diagram specifies the design of the engineered E. coli apFAB82 Test Cells which are transformed by the plasmid pSB1C3-BBa_K2832015.  The plasmid encodes the apFAB82 Test Device which contains the BIOFAB Promoter apFAB46. .  This device was submitted for the silver medal requirement.
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https://static.igem.org/mediawiki/parts/8/86/ApFAB82_Plasmid_Map.png
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pSB1C3-BBa_K2832015 Plasmid Map: The plasmid contains apFAB82 Test Device
  
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Plasmid map that includes BIOFAB promoter apFAB82
 
  
  

Latest revision as of 20:01, 2 December 2018


BIOFAB Modular Promoter apFAB82 Driven mRFP1 Generator ( ---> mRFP1 )


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 630
    Illegal AgeI site found at 742
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

ApFAB82_Design_Diagram.png apFAB82 Test Device: The diagram specifies the design of the engineered E. coli apFAB82 Test Cells which are transformed by the plasmid pSB1C3-BBa_K2832015. The plasmid encodes the apFAB82 Test Device which contains the BIOFAB Promoter apFAB46. . This device was submitted for the silver medal requirement. ApFAB82_Plasmid_Map.png pSB1C3-BBa_K2832015 Plasmid Map: The plasmid contains apFAB82 Test Device


Source

Source...

References

Mutalik,V. Guimaraes, J. Cambray, G. Lam, C. Christoffersen, M. Mai, Q. Tran, A Morgan Paull, Keasling, J. Arkin, A. Endy, D. (2013). Precise and reliable gene expression via standard transcription and translation initiation elements: Nature Methods, 354-360