Difference between revisions of "Part:BBa K2832004:Design"

 
 
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===Design Notes===
 
===Design Notes===
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Design considerations...
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https://static.igem.org/mediawiki/parts/5/58/PzntA_Design_Diagram.png
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PzntA Design Diagram. The zntA promoter sequence binds ZntR when activated by zinc. ZntR is a zinc-dependent promoter and detects increasing zinc concentration, possible through the opening of a mechanosensitive channel. mRFP1 signal indicates successful activation of the pathway.
  
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https://static.igem.org/mediawiki/parts/f/f7/PzntA_plasmid_map.png
  
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FSU Promoter BBa_K2832004 inside plasmid pSB1C3.
 
===Source===
 
===Source===
  

Latest revision as of 22:34, 1 December 2018


PzntA - Inducible Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 13
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PzntA_Design_Diagram.png PzntA Design Diagram. The zntA promoter sequence binds ZntR when activated by zinc. ZntR is a zinc-dependent promoter and detects increasing zinc concentration, possible through the opening of a mechanosensitive channel. mRFP1 signal indicates successful activation of the pathway.

PzntA_plasmid_map.png

FSU Promoter BBa_K2832004 inside plasmid pSB1C3.

Source

E. coli K12 genome.

References