Difference between revisions of "Part:BBa K2653004"

 
 
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We use this part to tag our trim21 so that it can be easily detected.
 
We use this part to tag our trim21 so that it can be easily detected.
  
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===Usage and Biology===
 
===Usage and Biology===
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HA-tag has long been used as the epitopic tag when it is hard to test whether a target gene has expressed or not. The HA-tag we use in our system has the length of 27 bps. By testing HA-tag with its antibody, we successfully validated the expression of trim21 which has hardly no epitopic sign to test. It is a really useful tool.
  
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===Sequence and Features===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2653004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2653004 SequenceAndFeatures</partinfo>
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===Functional Parameters===
 
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<partinfo>BBa_K2653004 parameters</partinfo>
 
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Latest revision as of 03:44, 18 October 2018


HA Tag

We use this part to tag our trim21 so that it can be easily detected.


Usage and Biology

HA-tag has long been used as the epitopic tag when it is hard to test whether a target gene has expressed or not. The HA-tag we use in our system has the length of 27 bps. By testing HA-tag with its antibody, we successfully validated the expression of trim21 which has hardly no epitopic sign to test. It is a really useful tool.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]