Difference between revisions of "Part:BBa K2549060"
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Revision as of 03:38, 18 October 2018
minimal CMV2
It is another version of minimal CMV which provides very low basal expression and high maximal expression after induction. Minimal CMV2 shows a better sensitivity of responsing to transcription regulation[1].
Biology
Loew R. et al stated Using both plasmid-based and retroviral gene delivery, our analysis revealed that especially background expression levels could be significantly reduced when compared to previously established "standard" promoter designs. Our results also demonstrate the possibility to fine-tune expression levels in non-clonal cell populations. They also imply differences regarding the requirements for tight regulation and high level induction between transient and stable gene transfer systems.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
- ↑ Loew R, Heinz N, Hampf M, Bujard H, Gossen M. Improved Tet-responsive promoters with minimized background expression. BMC Biotechnology. 2010;10:81. doi:10.1186/1472-6750-10-81.