Difference between revisions of "Part:BBa K2729011"
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As shown in Figure 1, the band of DENV2 prME come around 2,000 bp. | As shown in Figure 1, the band of DENV2 prME come around 2,000 bp. | ||
<br style="clear: both" /> | <br style="clear: both" /> | ||
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| + | ===Analysis=== | ||
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| + | Our team successfully validated that the sequence was synthesized, and obtained enough amount of it for the transfection. | ||
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| + | If it works well in the process of pseudo-virus production and also infection into host cells as expected, the fluorescence intensity can be observed robustly. | ||
==Sequence and Features== | ==Sequence and Features== | ||
Latest revision as of 03:34, 18 October 2018
DENV2 ancC-prM-E (Dengue Virus Serotype II)
Usage and Biology
Flavivirus Capsid (C), prM (precursor Membrane) and Envelope (E) are structural proteins responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.
Characterization
The prM protein consists of an N-terminal pr domain followed by the M protein separated by a furin cleavage site.(15) The pr part of the protein consists mainly of β-strands, whereas the M portion consists of a linear structure followed by a mainly α-helical stem region and two transmembrane helices.
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As shown in Figure 1, the band of DENV2 prME come around 2,000 bp.
Analysis
Our team successfully validated that the sequence was synthesized, and obtained enough amount of it for the transfection.
If it works well in the process of pseudo-virus production and also infection into host cells as expected, the fluorescence intensity can be observed robustly.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 946
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1397
Illegal AgeI site found at 1108
Illegal AgeI site found at 1450 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 349