Difference between revisions of "Part:BBa K2700002"
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The pT181-promoter and 5’ untranslated region enable the expression of a downstream gene. In the absence of the antisense transcript, the 5’ region folds to create the structure depicted in figure 1 [2]. In this conformation, both transcription and ribosomal binding are unhindered. | The pT181-promoter and 5’ untranslated region enable the expression of a downstream gene. In the absence of the antisense transcript, the 5’ region folds to create the structure depicted in figure 1 [2]. In this conformation, both transcription and ribosomal binding are unhindered. | ||
Latest revision as of 03:31, 18 October 2018
Promoter-RNA-mediated transcription/translation repression
The pT181-promoter and 5’ untranslated region enable the expression of a downstream gene. In the absence of the antisense transcript, the 5’ region folds to create the structure depicted in figure 1 [2]. In this conformation, both transcription and ribosomal binding are unhindered.
In the presence of the antisense transcript, the 5’ region folds to create the structure depicted in figure 2 [2]. In this conformation, two hairpin structures are formed. The first hairpin structure functions as a premature transcriptional terminator, regulating expression at the transcriptional level. The second hairpin structure is formed at the RBS, regulating expression at a translational level. This dual regulation enabled a 98% repression a RFP expression upon induction of the antisense transcript [2].
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]