Difference between revisions of "Part:BBa K2700002"

 
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<partinfo>BBa_K2700002 short</partinfo>
 
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The pT181-promoter and 5’ untranslated region enable the expression of a downstream gene.  In the absence of the antisense transcript, the 5’ region folds to create the structure depicted in figure 1 [2].  In this conformation, both transcription and ribosomal binding are unhindered.
  
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In the presence of the antisense transcript, the 5’ region folds to create the structure depicted in figure 2 [2].  In this conformation, two hairpin structures are formed.  The first hairpin structure functions as a premature transcriptional terminator, regulating expression at the transcriptional level.  The second hairpin structure is formed at the RBS, regulating expression at a translational level.  This dual regulation enabled a 98% repression a RFP expression upon induction of the antisense transcript [2].
  
 
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Revision as of 03:31, 18 October 2018


Promoter-RNA-mediated transcription/translation repression The pT181-promoter and 5’ untranslated region enable the expression of a downstream gene. In the absence of the antisense transcript, the 5’ region folds to create the structure depicted in figure 1 [2]. In this conformation, both transcription and ribosomal binding are unhindered.

In the presence of the antisense transcript, the 5’ region folds to create the structure depicted in figure 2 [2]. In this conformation, two hairpin structures are formed. The first hairpin structure functions as a premature transcriptional terminator, regulating expression at the transcriptional level. The second hairpin structure is formed at the RBS, regulating expression at a translational level. This dual regulation enabled a 98% repression a RFP expression upon induction of the antisense transcript [2].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]