Difference between revisions of "Part:BBa K2653000:Design"

 
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===Design Notes===
 
===Design Notes===
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There is 2 domains for PstI with the sequence of our Trim21, so we carried out point mutation to avoid this.
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Trim21 has a special crab-shaped domain that can bind with the B30.2 domain of the Fc. Thus, with conservative Fc of the antibody binding with trim21, it then functions as a E3 ubiquitin ligase and proceeds the ubiquitin process of the antibody-antigen complex. Afterwards the proteasome depletes the target protein along with its antibody.
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===Source===
 
===Source===

Revision as of 03:26, 18 October 2018


trim21


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 204
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 873
    Illegal BamHI site found at 1411
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 161
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1251


Design Notes

Trim21 has a special crab-shaped domain that can bind with the B30.2 domain of the Fc. Thus, with conservative Fc of the antibody binding with trim21, it then functions as a E3 ubiquitin ligase and proceeds the ubiquitin process of the antibody-antigen complex. Afterwards the proteasome depletes the target protein along with its antibody.

Source

Its sequence come from articles.

References