Difference between revisions of "Part:BBa K2859000:Experience"
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| − | We synthesised the part flanked by bioBrick prefix and suffix. Then, the synthesized gblock was ligated into pSB1C3 by restriction digest with EcoR1 and Pst1 and transformed into E.Coli DH5-α.We confirmed that the ligation was successfully done by running gel with minicprepped DNA cut with EcoR1 (about 2. | + | We synthesised the part flanked by bioBrick prefix and suffix. Then, the synthesized gblock was ligated into pSB1C3 by restriction digest with EcoR1 and Pst1 and transformed into E.Coli DH5-α.We confirmed that the ligation was successfully done by running gel with minicprepped DNA cut with EcoR1 (about 2.5kb). The transformed E.coli was grown at 37°C. The expression of Penetratin-Insulin-Flag tag complex was evaluated by SDS page gel. |
| + | https://static.igem.org/mediawiki/2018/e/ef/T--HAFS--Result_Gel.png" | ||
Figure1. 1.2% Agarose Gel electrophoresis result of pSB1C3 cut with EcoR1 | Figure1. 1.2% Agarose Gel electrophoresis result of pSB1C3 cut with EcoR1 | ||
https://res.cloudinary.com/dt6jjveot/image/upload/SDS.jpg | https://res.cloudinary.com/dt6jjveot/image/upload/SDS.jpg | ||
| + | |||
| + | Figure2. 10% SDS page gel result showing the expression of protein | ||
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We synthesised the part flanked by bioBrick prefix and suffix. Then, the synthesized gblock was ligated into pSB1C3 by restriction digest with EcoR1 and Pst1 and transformed into E.Coli DH5-α.We confirmed that the ligation was successfully done by running gel with minicprepped DNA cut with EcoR1 (about 2.5kb). The transformed E.coli was grown at 37°C. The expression of Penetratin-Insulin-Flag tag complex was evaluated by SDS page gel.
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Figure1. 1.2% Agarose Gel electrophoresis result of pSB1C3 cut with EcoR1
Figure2. 10% SDS page gel result showing the expression of protein