Difference between revisions of "Part:BBa K2859002:Experience"
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We synthesised the part flanked by bioBrick prefix and suffix. Then, the synthesized gblock was ligated into pSB1C3 by restriction digest with EcoR1 and Pst1 and transformed into E.Coli DH5-α.We confirmed that the ligation was successfully done by running gel with minicprepped DNA cut with EcoR1 (about 2.5kb). The transformed E.coli was grown at 37°C. The expression of Penetratin-Insulin-Flag tag complex was evaluated by SDS page gel.
 
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Figure1. 1.2% Agarose Gel electrophoresis result of pSB1C3 cut with EcoR1
 
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https://res.cloudinary.com/dt6jjveot/image/upload/SDS.jpg
 
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Figure2. 10% SDS page gel result showing protein expression
 

Latest revision as of 03:16, 18 October 2018


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