Difference between revisions of "Part:BBa K914009:Experience"

Latest revision as of 03:04, 18 October 2018

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K914009

The leakiness of the Kan* gene is higher than expected, and thus one mutation is not sufficient for the containment. Indeed, at usual concentration, Kan* gene manages to express an antibiotic resistance, even though lower than Kan^R or supD (Figure 4A). Here we define the growth rate as the OD₆₀₀ observed at a given time (here t=8h20'), in order to overcome the fact that Kan*+RFP does not have a clear exponential phase. The fact that the strain (3) grows faster and can have higher OD₆₀₀ may be explain by the fact that supD is deleterious for the strain, by removing some stop codon of other genes for instance.

Figure 4A and 4B: The bar graph represent the OD₆₀₀ at time = 8h20' (black line Figure 4C) for the different strains at a given kanamycin concentration (black line on Figure 4B). On B, we observe the variations of the OD₆₀₀ at different kanamycin concentrations, at the same time.

Figure 4C: The variations of the OD₆₀₀ is observed in function of the time, for different concentrations of kanamycin (400µg/mL, 100µg/mL, 25µg/mL).

This part has been further characterised and improved by Edinburgh UG 2018:

http://2018.igem.org/Team:Edinburgh_UG/Results

User Reviews

UNIQbc345a288412e951-partinfo-00000000-QINU UNIQbc345a288412e951-partinfo-00000001-QINU