Difference between revisions of "Part:BBa K2719008:Design"
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===References===
 
===References===
 +
<p>M.N.M Walter. et al (2010).<i> Mesenchymal stem cell-conditioned medium accelerates skin wound healing: An in vitro study of fibroblast and keratinocyte scratch assays</i>. Doi:10:1016 /j.excr.2010.02.26</p>
 +
<p>Ahsan SM. et al (2017).<i> Chitosan as biomaterial in drug delivery and tissue engineering </i>doi: 10.1016/j.ijbiomac.2017.08.140.</p>
 +
<p>Gainsford T. et al (1998). <i>Leptin can induce proliferation, differentiation, and functional activation of hemopoietic cells.</i> 10;93(25):14564-8.</p>

Revision as of 03:03, 18 October 2018


Leptin

This is originated from Hommo sapiens, the original sequence is from this species but was optimized for E.coli BL21 (DE3) and to be cloned by RFC10 on pSB1C3. It mainly consists on a-helix. An histidine tag (6xHis) was added to the protein to facilitate its purification.

"T--TecCEM--Lep3DStructure.png"

Figure 7. Leptin 3D prediction modelled with Swiss Model


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Optimized for E.coli.


Source

NCBI Genomic Library

References

M.N.M Walter. et al (2010). Mesenchymal stem cell-conditioned medium accelerates skin wound healing: An in vitro study of fibroblast and keratinocyte scratch assays. Doi:10:1016 /j.excr.2010.02.26

Ahsan SM. et al (2017). Chitosan as biomaterial in drug delivery and tissue engineering doi: 10.1016/j.ijbiomac.2017.08.140.

Gainsford T. et al (1998). Leptin can induce proliferation, differentiation, and functional activation of hemopoietic cells. 10;93(25):14564-8.