Difference between revisions of "Part:BBa K2575001:Design"

 
 
Line 2: Line 2:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2575001 short</partinfo>
 
<partinfo>BBa_K2575001 short</partinfo>
−
 
+
Due to repetitive sequences, the part had to be cloned out of pJAB 1504 using primers to add iGEM restriction sites.
 
<partinfo>BBa_K2575001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2575001 SequenceAndFeatures</partinfo>
  

Latest revision as of 02:59, 18 October 2018


6X UAS-35S minimal promoter Due to repetitive sequences, the part had to be cloned out of pJAB 1504 using primers to add iGEM restriction sites.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 208
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 208
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 208
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 208
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR out of vector, not able to be synthesized in 1 unit


Source

Synthetic/minimal promoter from cauliflower mosaic virus design

References