Difference between revisions of "Part:BBa K2638998"

 
 
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<partinfo>BBa_K2638998 short</partinfo>
 
<partinfo>BBa_K2638998 short</partinfo>
  
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For production of the mutated Ferritin the araBAD promotor with RBS was put in front of the gene.
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AhpF belongs to the family of pyridine nucleotide-disulphide oxidoreductases and channels electrons from NAD(P)H towards the AhpC (https://parts.igem.org/Part:BBa_K2638100, BBa_K2638100) component which is able to reduce hydrogen peroxide and organic hydroperoxides. AhpF forms together with AhpC the holoenzyme Ahp / AhpR. The protein can bind NADH or NADPH in order to gain electrons for the reduction of oxidized AhpC. The active site is a redox-active disulfide bond. FAD is needed as a cofactor.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 02:43, 18 October 2018


alkyl hydroperoxide reductase, F52a subunit (ahpF)

AhpF belongs to the family of pyridine nucleotide-disulphide oxidoreductases and channels electrons from NAD(P)H towards the AhpC (https://parts.igem.org/Part:BBa_K2638100, BBa_K2638100) component which is able to reduce hydrogen peroxide and organic hydroperoxides. AhpF forms together with AhpC the holoenzyme Ahp / AhpR. The protein can bind NADH or NADPH in order to gain electrons for the reduction of oxidized AhpC. The active site is a redox-active disulfide bond. FAD is needed as a cofactor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 289
    Illegal AgeI site found at 712
    Illegal AgeI site found at 964
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 613