Difference between revisions of "Part:BBa K2876015"
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This sequence produces a single-chain antibody that targets the immunoprotein interleukin B1 (BBa_K2876014) (https://parts.igem.org/Part:BBa_K2876014). We fused tthis antibody to the alpha subunit of RNA-polymerase III (BBa_K2876002) (https://parts.igem.org/Part:BBa_K2876002) described in Dove, Joung, and Hochschild in order to a fusion protein capable of activating transcription in the presence of IL1B and another antibody fusion protein. For our reporter we used the previously described pOL2-62 from Dove, Joung, and Hochschild fused to a RFP (BBa_K2876000) (https://parts.igem.org/Part:BBa_K2876000). | This sequence produces a single-chain antibody that targets the immunoprotein interleukin B1 (BBa_K2876014) (https://parts.igem.org/Part:BBa_K2876014). We fused tthis antibody to the alpha subunit of RNA-polymerase III (BBa_K2876002) (https://parts.igem.org/Part:BBa_K2876002) described in Dove, Joung, and Hochschild in order to a fusion protein capable of activating transcription in the presence of IL1B and another antibody fusion protein. For our reporter we used the previously described pOL2-62 from Dove, Joung, and Hochschild fused to a RFP (BBa_K2876000) (https://parts.igem.org/Part:BBa_K2876000). | ||
Revision as of 02:35, 18 October 2018
anti-IL1B scFv1
This sequence produces a single-chain antibody that targets the immunoprotein interleukin B1 (BBa_K2876014) (https://parts.igem.org/Part:BBa_K2876014). We fused tthis antibody to the alpha subunit of RNA-polymerase III (BBa_K2876002) (https://parts.igem.org/Part:BBa_K2876002) described in Dove, Joung, and Hochschild in order to a fusion protein capable of activating transcription in the presence of IL1B and another antibody fusion protein. For our reporter we used the previously described pOL2-62 from Dove, Joung, and Hochschild fused to a RFP (BBa_K2876000) (https://parts.igem.org/Part:BBa_K2876000). Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 255
Illegal PstI site found at 288 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 255
Illegal PstI site found at 288
Illegal NotI site found at 12 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 756
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 255
Illegal PstI site found at 288 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 255
Illegal PstI site found at 288 - 1000COMPATIBLE WITH RFC[1000]