Difference between revisions of "Part:BBa K2524000"
| Line 32: | Line 32: | ||
Annealing Temperature: 64℃ | Annealing Temperature: 64℃ | ||
| − | Optimized primer for HCG for pGEX | + | Optimized primer for HCG for pGEX <br> |
| − | Name: new hcg reverse for pGEX | + | Name: new hcg reverse for pGEX <br> |
| − | Sequence: CGACGTACGGTCgcggccgcTCACTGAGGAAGAATTGGGGTG | + | Sequence: CGACGTACGGTCgcggccgcTCACTGAGGAAGAATTGGGGTG <br> |
| − | Annealing Temperature: 70℃ | + | Annealing Temperature: 70℃ <br> |
| − | Name: HCG for pGEX F | + | Name: HCG for pGEX F <br> |
| − | Sequence: cccaatttagaattcgATGGAAATGTTCCAGGGGCT | + | Sequence: cccaatttagaattcgATGGAAATGTTCCAGGGGCT <br> |
| − | Annealing Temperature: 62℃ | + | Annealing Temperature: 62℃ <br> |
Revision as of 02:34, 18 October 2018
Synthetic Human Chorionic Gonadotropin (HCG) Beta Subunit
This part is an improvement of Part:BBa_K732001.
Human Chorionic Gonadotropin (hCG) is the hormone made by chorionic cells in the fetal part of the placenta where it stimulates the ovaries to synthesize important steroids for the maintenance of pregnancy. It is a member of the beta chain glycoprotein hormone family. The glycoproteins are heterodimers consisting of an alpha subunit and beta subunit. The beta subunit determines bio-specificity. Six genes encode the hCG beta subunit which are inverted on chromosome 19q13.3 and is boarding the luteinizing hormone beta subunit gene.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Optimized primers for HCG for pSB1C3 Name: HCG New Prefix Primer 1 Sequence: agtcgaattcgcggccgcttctagATGGAAATGTTCCAGGGGCT Annealing Temperature: 56℃ Name: New HCG Suffix Sequence: GCAACTGCAGCGGCCGCTACTAGTATCACTGAGGAAGAATTGGGGTG Annealing Temperature: 64℃ Optimized primer for HCG for pGEX
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