Difference between revisions of "Part:BBa K2653000"

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===Source===
 
===Source===
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The sequence of trim21 comes from the article: James L C, Keeble A H, Khan Z, et al. Structural basis for PRYSPRY-mediated tripartite motif (TRIM) protein function[J]. Proceedings of the National Academy of Sciences of the United States of America, 2007, 104(15):6200-6205.  
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The sequence of Trim21 is from the NCBI database.
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<partinfo>BBa_K2653000 parameters</partinfo>
 
<partinfo>BBa_K2653000 parameters</partinfo>
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==通常==
 
==通常==

Revision as of 02:32, 18 October 2018


trim21

Trim21 is a type of E3 ubiquitin ligase that has been discovered in many eukaryotic cells.

Usage and Biology

The Tripartite motif-containing protein 21(Trim21) belongs to the E3 ubiquitin ligase family, which is encoded by the TRIM21 gene.The C-terminal B30.2 domain on trim21 shapes like a crab and offers a site for the conservative Fc region of human IgG 1,2 and 4 to bind with. This makes trim21 a potential protein degradation tool---when it binds with hIgG1-Fc, and after the antibody-antigen interaction, a ternary trim21-antibody-antigen complex would be built up. The trim21 then functions as a E3 ubiquitin ligase and proceeds the complex to be depleted through the ubiquitin-proteasome pathway.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 204
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 873
    Illegal BamHI site found at 1411
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 161
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1251

Source

The sequence of trim21 comes from the article: James L C, Keeble A H, Khan Z, et al. Structural basis for PRYSPRY-mediated tripartite motif (TRIM) protein function[J]. Proceedings of the National Academy of Sciences of the United States of America, 2007, 104(15):6200-6205.

通常

There are 2 domains for PstI with the sequence of our Trim21, so we carried out point mutation to avoid this.


Source

Its sequence come from articles.

References