Difference between revisions of "Part:BBa K2524000"
Line 22: Line 22:
  
 
</tr>
 
</tr>
−
</table>jpg
+
</table>
  
 +
Optimized primers for HCG for pSB1C3
 +
Name: HCG New Prefix Primer 1
 +
Sequence: agtcgaattcgcggccgcttctagATGGAAATGTTCCAGGGGCT
 +
Annealing Temperature: 56℃
 +
Name: New HCG Suffix
 +
Sequence: GCAACTGCAGCGGCCGCTACTAGTATCACTGAGGAAGAATTGGGGTG
 +
Annealing Temperature: 64℃
 +
 +
Optimized primer for HCG for pGEX
 +
Name: new hcg reverse for pGEX
 +
Sequence: CGACGTACGGTCgcggccgcTCACTGAGGAAGAATTGGGGTG
 +
Annealing Temperature: 70℃
 +
Name: HCG for pGEX F
 +
Sequence: cccaatttagaattcgATGGAAATGTTCCAGGGGCT
 +
Annealing Temperature: 62℃
  
  

Revision as of 02:31, 18 October 2018


Synthetic Human Chorionic Gonadotropin (HCG) Beta Subunit This part is an improvement of Part:BBa_K732001. Human Chorionic Gonadotropin (hCG) is the hormone made by chorionic cells in the fetal part of the placenta where it stimulates the ovaries to synthesize important steroids for the maintenance of pregnancy. It is a member of the beta chain glycoprotein hormone family. The glycoproteins are heterodimers consisting of an alpha subunit and beta subunit. The beta subunit determines bio-specificity. Six genes encode the hCG beta subunit which are inverted on chromosome 19q13.3 and is boarding the luteinizing hormone beta subunit gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

A

T--Georgia_State--hcgbetasubunit.jpg


Optimized primers for HCG for pSB1C3 Name: HCG New Prefix Primer 1 Sequence: agtcgaattcgcggccgcttctagATGGAAATGTTCCAGGGGCT Annealing Temperature: 56℃ Name: New HCG Suffix Sequence: GCAACTGCAGCGGCCGCTACTAGTATCACTGAGGAAGAATTGGGGTG Annealing Temperature: 64℃

Optimized primer for HCG for pGEX Name: new hcg reverse for pGEX Sequence: CGACGTACGGTCgcggccgcTCACTGAGGAAGAATTGGGGTG Annealing Temperature: 70℃ Name: HCG for pGEX F Sequence: cccaatttagaattcgATGGAAATGTTCCAGGGGCT Annealing Temperature: 62℃