Difference between revisions of "Part:BBa K914009"
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The single mutation avoid the expression of the P1003 gene, that prevent the expression of a kanamycin resistance. | The single mutation avoid the expression of the P1003 gene, that prevent the expression of a kanamycin resistance. | ||
| − | This mutation is rescue with the tRNA amber suppressor supD (BBa_K914000), or any genotype relative to sup44 . The idea here is to prevent the kanamycin gene resistance in case horizontal gene transfer. | + | This mutation is rescue with the tRNA amber suppressor supD (BBa_K914000 - https://parts.igem.org/Part:BBa_K914000), or any genotype relative to sup44 . The idea here is to prevent the kanamycin gene resistance in case horizontal gene transfer. |
Actually it would be better with another mutation. | Actually it would be better with another mutation. | ||
Revision as of 02:24, 18 October 2018
P1003* Ser133->Amber Codon
The single mutation avoid the expression of the P1003 gene, that prevent the expression of a kanamycin resistance. This mutation is rescue with the tRNA amber suppressor supD (BBa_K914000 - https://parts.igem.org/Part:BBa_K914000), or any genotype relative to sup44 . The idea here is to prevent the kanamycin gene resistance in case horizontal gene transfer. Actually it would be better with another mutation.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]