Difference between revisions of "Part:BBa K2610040"

 
 
Line 3: Line 3:
 
<partinfo>BBa_K2610040 short</partinfo>
 
<partinfo>BBa_K2610040 short</partinfo>
  
−
Promoter GapA is naturally occuring constitutive promoter in Escherichia coli.  
+
Promoter GapA is naturally occuring constitutive promoter in <i> Escherichia coli </i>.  
  
−
 
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
 +
During our project, we isolated the GapA promoter from the <i> E. coli </i> genome. In contrast to many synthetic promoters, this novel promoter provides an indication of high levels of natural expression. Therefore, this promoter can be used as a future positive control of reporter production - such as GFP - in a natural situation. Moreover, GapA being a natural occurring promoter means that there are still possibilities for optimization through radiation. This way variations of this promoter can provide access to an even wider spectrum of colors.
 +
 +
In our project, we combined this naturally occurring constitutive promoter both with the blue chromoprotein AmilCP and the pink chromoprotein spisPink, creating pGapA-AmilCP ([https://parts.igem.org/Part:BBa_K2610041 BBa_K2610041]) pGapA-spisPink ([https://parts.igem.org/Part:BBa_K2610042 BBa_K2610042]). These constructs allowed us to compare natural high expression to the responses of our novel stress promoter+chromoprotein constructs.
 +
 +
 +
[[File:T--Leiden--chromo.png|600px]]
 +
 +
<span style="font-size:1em"><b>Figure 1.</b> Constitutively expressed pGapA-AmilCP compared to non-transformed DH5a and pGapA-spis-Pink (pink chromoprotein)</span>
 +
  
 
<!-- -->
 
<!-- -->

Latest revision as of 02:04, 18 October 2018


pGapA

Promoter GapA is naturally occuring constitutive promoter in Escherichia coli .

Usage and Biology

During our project, we isolated the GapA promoter from the E. coli genome. In contrast to many synthetic promoters, this novel promoter provides an indication of high levels of natural expression. Therefore, this promoter can be used as a future positive control of reporter production - such as GFP - in a natural situation. Moreover, GapA being a natural occurring promoter means that there are still possibilities for optimization through radiation. This way variations of this promoter can provide access to an even wider spectrum of colors.

In our project, we combined this naturally occurring constitutive promoter both with the blue chromoprotein AmilCP and the pink chromoprotein spisPink, creating pGapA-AmilCP (BBa_K2610041) pGapA-spisPink (BBa_K2610042). These constructs allowed us to compare natural high expression to the responses of our novel stress promoter+chromoprotein constructs.


T--Leiden--chromo.png

Figure 1. Constitutively expressed pGapA-AmilCP compared to non-transformed DH5a and pGapA-spis-Pink (pink chromoprotein)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 140