Difference between revisions of "Part:BBa K2560034:Design"
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===Source===
 
===Source===
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As source we used the part <a href="https://parts.igem.org/Part:BBa_B0010">BBa_B0010</a>.
 
As source we used the part <a href="https://parts.igem.org/Part:BBa_B0010">BBa_B0010</a>.
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Latest revision as of 01:44, 18 October 2018


Phytobrick version of BBa_B0010


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was created by Overlap extension polymerase chain reaction and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly.

Forward oligo: AACGTCTCGCTCGGCTTAACCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCC

Reverse Oligo: TTCGTCTCCCTCAAGCGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTG


Source

As source we used the part BBa_B0010.