Difference between revisions of "Part:BBa K2656106"

Revision as of 01:32, 18 October 2018

GFP transcriptional unit 2

Transcriptional unit assembled with a one-pot [http://2018.igem.org/Team:Valencia_UPV/Design Level 1] Golden Gate reaction using BsaI type IIS endonuclease (Figure 1).

Figure 1. Assembled transcriptional unit. Golden Gate scars: standardized overhangs for promoter, RBS, CDS and terminator

It is a composite part of our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection#com BioArt DNA toolkit palette: Printone ], as it can be used to express a green color with medium intensity.

This transcriptional unit is composed of the following standardized parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:

BBa_K2656005: the J23102 constitutive promoter in its Golden Braid compatible version
BBa_K2656009: the J61100 strong ribosome biding site in its Golden Braid compatible version
BBa_K2656022: the BBa_K2656022 GFPmut3b sequence in its Golden Braid standardized version
Terminator BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 11
Illegal NheI site found at 34
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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-This transcriptional unit has been used to characterize the constitutive [https://parts.igem.org/Part:BBa_K2656005 BBa_K2656005 promoter]. Following this [http://2018.igem.org/Team:Valencia_UPV/Modeling#http://2018.igem.org/Team:Valencia_UPV/Experiments#exp_protocol experimental protocol], we have obtained the parameters to valide our  [http://2018.igem.org/Team:Valencia_UPV/Modeling#models constitutive model] and so the results of the next graph have been obtained after an [http://2018.igem.org/Team:Valencia_UPV/Modeling#optimization optimization] and decision-making process, in which the optimal parameters have been selected and the constituent model for each objective has been simulated and compared with the experimental data.
-[[File:T--Valencia_UPV_promoter_UPV2018.png|900px|thumb|none|alt=Promoter experiment.|Figure 1. Promoter expression experiment with composite parts BBa_K2656105, BBa_K2656106 and BBa_K2656107]]
-{|class='wikitable'
-|colspan=4|Table 1. Optimized parameters for the BBa_K2656105.
-|-
-|'''Parameter'''
-|'''Value'''
-|-
-|Constitutive transcription rate CR
-|CR = 171.15 min-1
-|-
-| Dilution rate μ
-|μ = 0.0058 min-1
-|-
-|}