Difference between revisions of "Part:BBa K2804005"
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<partinfo>BBa_K2804005 short</partinfo>
 
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The superfolder green fluorescent protein (sfGFP) is fused to the C-terminus of the cellulose binding domain (CBD) of the scaffolding protein cipA of Clostridium thermocellum.
 
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===Usage and Biology===
 
===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K2804005 SequenceAndFeatures</partinfo>
 
  
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<p align="justify">The Microbial Fuel Cell (MFC) can be a future environmentally friendly biotechnological application for the production of electrical energy. As a future alternative energy source, the bioelectricity generation must become more efficient. A major limiting factor is the low bacterial membrane permeability, hindering transport of electron shuttles through the membrane and thereby restricting the electron shuttle-mediated extracellular electron transfer (EET) from bacteria to electrodes. This results in a reduced electrical power output of the MFC. Therefore, we heterologously expressed the porin protein OprF from ''Pseudomonas fluorescens'' into ''Escherichia coli''.</p>
  
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<img src="https://static.igem.org/mediawiki/2018/e/e4/T--Ecuador--CBD-sfGFP.png" width="268" height="261"/>Figure 1: Schematic overview of the enhancement mechanism of electron shuttle-mediated electron transfer between bacteria and the anode of MFCs by the synthetic porin OprF. Oxidized mediators diffuse into the periplasmatic space where they accept electrons. Reduced mediators are secreted through outer membrane porins and donate their electrons to the electrode.'''</p>]]
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===Functional Parameters===
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<partinfo>BBa_K2804005 parameters</partinfo>
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Revision as of 01:29, 18 October 2018


CBD cipA fused to sfGFP under the control of LacI promoter

Usage and Biology

The Microbial Fuel Cell (MFC) can be a future environmentally friendly biotechnological application for the production of electrical energy. As a future alternative energy source, the bioelectricity generation must become more efficient. A major limiting factor is the low bacterial membrane permeability, hindering transport of electron shuttles through the membrane and thereby restricting the electron shuttle-mediated extracellular electron transfer (EET) from bacteria to electrodes. This results in a reduced electrical power output of the MFC. Therefore, we heterologously expressed the porin protein OprF from Pseudomonas fluorescens into Escherichia coli.

<img src="T--Ecuador--CBD-sfGFP.png" width="268" height="261"/>Figure 1: Schematic overview of the enhancement mechanism of electron shuttle-mediated electron transfer between bacteria and the anode of MFCs by the synthetic porin OprF. Oxidized mediators diffuse into the periplasmatic space where they accept electrons. Reduced mediators are secreted through outer membrane porins and donate their electrons to the electrode.</p>]]