Difference between revisions of "Part:BBa K2534022:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2534022 short</partinfo>
 
<partinfo>BBa_K2534022 short</partinfo>
 +
[http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] created BBa K2534022 tetracycline Resistance gene as an efficient switching control system for lentiviral delivery of mIRNAs into Colorectal cancer cell line RKO. This comes in a trial to to improve tetR registry part BBa_K182005 which lacks a terminator We also worked to improve this part by adding a universal terminator To improve the control of the highly specific interaction of the tet repressor protein (TetR) with (tetO). TetR binding on tetO classically stops expression of genes downstream of tetO by excluding RNA polymerase from binding the promoter and initiating transcription.
 +
[http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] have characterized this part considering it's construction into the transfer vector of the lentiviral delivery.
  
 +
For further information about demonstration of our results, please visit our results page
 +
http://2018.igem.org/Team:AFCM-Egypt/Demonstrate
 +
For further information about design of our parts, please visit our Parts & Design Page
 +
http://2018.igem.org/Team:AFCM-Egypt/Parts
 
<partinfo>BBa_K2534022 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2534022 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
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IDT
+
This part has been synthesized through IDT
  
  
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===Source===
 
===Source===
  
−
IDT
+
[http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] created BBa K2534022 tetracycline Resistance gene as an efficient switching control system for lentiviral delivery of mIRNAs into Colorectal cancer cell line RKO. This comes in a trial to to improve tetR registry part BBa_K182005 which lacks a terminator We also worked to improve this part by adding a universal terminator To improve the control of the highly specific interaction of the tet repressor protein (TetR) with (tetO). TetR binding on tetO classically stops expression of genes downstream of tetO by excluding RNA polymerase from binding the promoter and initiating transcription.
 +
[http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] have characterized this part considering it's construction into the transfer vector of the lentiviral delivery.
  
−
===References===
+
For further information about demonstration of our results, please visit our results page
 +
http://2018.igem.org/Team:AFCM-Egypt/Demonstrate
 +
For further information about design of our parts, please visit our Parts & Design Page
 +
http://2018.igem.org/Team:AFCM-Egypt/Parts===References===

Revision as of 01:15, 18 October 2018


TetR [http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] created BBa K2534022 tetracycline Resistance gene as an efficient switching control system for lentiviral delivery of mIRNAs into Colorectal cancer cell line RKO. This comes in a trial to to improve tetR registry part BBa_K182005 which lacks a terminator We also worked to improve this part by adding a universal terminator To improve the control of the highly specific interaction of the tet repressor protein (TetR) with (tetO). TetR binding on tetO classically stops expression of genes downstream of tetO by excluding RNA polymerase from binding the promoter and initiating transcription. [http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] have characterized this part considering it's construction into the transfer vector of the lentiviral delivery.

For further information about demonstration of our results, please visit our results page http://2018.igem.org/Team:AFCM-Egypt/Demonstrate For further information about design of our parts, please visit our Parts & Design Page http://2018.igem.org/Team:AFCM-Egypt/Parts


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has been synthesized through IDT


Source

[http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] created BBa K2534022 tetracycline Resistance gene as an efficient switching control system for lentiviral delivery of mIRNAs into Colorectal cancer cell line RKO. This comes in a trial to to improve tetR registry part BBa_K182005 which lacks a terminator We also worked to improve this part by adding a universal terminator To improve the control of the highly specific interaction of the tet repressor protein (TetR) with (tetO). TetR binding on tetO classically stops expression of genes downstream of tetO by excluding RNA polymerase from binding the promoter and initiating transcription. [http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] have characterized this part considering it's construction into the transfer vector of the lentiviral delivery.

For further information about demonstration of our results, please visit our results page http://2018.igem.org/Team:AFCM-Egypt/Demonstrate For further information about design of our parts, please visit our Parts & Design Page http://2018.igem.org/Team:AFCM-Egypt/Parts===References===