Difference between revisions of "Part:BBa K2804005"
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The superfolder green fluorescent protein (sfGFP) is fused to the C-terminus of the cellulose binding domain (CBD) of the scaffolding protein cipA of Clostridium thermocellum. | The superfolder green fluorescent protein (sfGFP) is fused to the C-terminus of the cellulose binding domain (CBD) of the scaffolding protein cipA of Clostridium thermocellum. | ||
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<p><img src="https://static.igem.org/mediawiki/2018/e/e4/T--Ecuador--CBD-sfGFP.png" width="268" height="261"/></p> | <p><img src="https://static.igem.org/mediawiki/2018/e/e4/T--Ecuador--CBD-sfGFP.png" width="268" height="261"/></p> | ||
<p><strong>Figure 2. </strong>Tridimensional structure of CBD cipA-sfGFP obtained from the server I-Tasser.</p> | <p><strong>Figure 2. </strong>Tridimensional structure of CBD cipA-sfGFP obtained from the server I-Tasser.</p> | ||
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Revision as of 01:12, 18 October 2018
CBD cipA fused to sfGFP under the control of LacI promoter
The superfolder green fluorescent protein (sfGFP) is fused to the C-terminus of the cellulose binding domain (CBD) of the scaffolding protein cipA of Clostridium thermocellum.
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<a name="SF">CBD cipA-sfGFP</a>
<img src="
" width="268" height="261"/>
Figure 2. Tridimensional structure of CBD cipA-sfGFP obtained from the server I-Tasser.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 230
Illegal AgeI site found at 1493 - 1000COMPATIBLE WITH RFC[1000]