Difference between revisions of "Part:BBa K2804005"
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The superfolder green fluorescent protein (sfGFP) is fused to the C-terminus of the cellulose binding domain (CBD) of the scaffolding protein cipA of Clostridium thermocellum.
 
The superfolder green fluorescent protein (sfGFP) is fused to the C-terminus of the cellulose binding domain (CBD) of the scaffolding protein cipA of Clostridium thermocellum.
  
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===Usage and Biology===
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                     <p><img src="https://static.igem.org/mediawiki/2018/e/e4/T--Ecuador--CBD-sfGFP.png" width="268" height="261"/></p>
 
                     <p><img src="https://static.igem.org/mediawiki/2018/e/e4/T--Ecuador--CBD-sfGFP.png" width="268" height="261"/></p>
 
                       <p><strong>Figure 2. </strong>Tridimensional structure of CBD cipA-sfGFP  obtained from the server I-Tasser.</p>
 
                       <p><strong>Figure 2. </strong>Tridimensional structure of CBD cipA-sfGFP  obtained from the server I-Tasser.</p>
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  <p>The gBlocks synthetized for in-frame protein assembly corresponded to the first gBlock consisting of lacI promoter, RBS and CDS of CBD cipA; the second gBlock containing the CDS of sfGFP with a 6X His tag. Both of them were codon optimized for <em>E</em>. <em>coli</em>.</p>
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  <p>We aimed to add a 6X His tag at C-terminus in order to visualize and measure at single-molecule level the interaction between the CBD cipA and bacterial cellulose.<sup>6</sup></p>
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<p><center><img src="https://static.igem.org/mediawiki/2018/5/5b/T--Ecuador--CBD_cipA-sfGFP_construct_Map.png" width="450" height="450"/></center></p><br>
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 01:09, 18 October 2018


CBD cipA fused to sfGFP under the control of LacI promoter

The superfolder green fluorescent protein (sfGFP) is fused to the C-terminus of the cellulose binding domain (CBD) of the scaffolding protein cipA of Clostridium thermocellum. 

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<a name="SF">CBD cipA-sfGFP</a>

<img src="T--Ecuador--CBD-sfGFP.png" width="268" height="261"/>

Figure 2. Tridimensional structure of CBD cipA-sfGFP obtained from the server I-Tasser.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 230
    Illegal AgeI site found at 1493
  • 1000
    COMPATIBLE WITH RFC[1000]